Sample Processing

Our facility provides expertise and quality services to provide optimal pre-analytical samples

About

Underpinning outstanding and reproducible science, are consistent and high-quality samples. The quality of these samples is largely dependent on pre-analytical conditions including the timing, collection, storage conditions and sample preparation techniques used. The consistency of producing these samples is paramount in producing consistent and meaningful data.

The Sample Processing Service provides expertise and service for the pre-analytical sample handling and preparation of biological samples and is designed to efficiently and economically meet requirements. The service works alongside researchers and clients, to design pre-analytical sample handling solutions for the collection, transportation, storage, aliquoting, blood fractioning and cell isolation (including PBMC preparation and storage), nucleic extraction (DNA and RNA extraction), nucleic sample QC, and PCR of samples.

Capacity for high-throughput processing or single sample processing is accommodated. Our Service dovetails a range of the Scientific Services in particular, working closer with the Sequencing Service, to provide a seamless product from sample collection to data.

Services

Advice for pre-analytical sample handling, design and project logistics.

Sample accession and storage in LIMS system.

Pre-analytical processing and handling of biologicals.

Blood fractioning and storage, including cell isolation using density gradient centrifugation (e.g. PBMC isolation).

Nucleic extraction (high-throughput and individualised) from a variety of sources including:

  • blood and blood products – whole blood, buffy coat, circulating cell-free fluids
  • saliva – collection kit, fresh
  • tissue – fresh, frozen and formalin fixed paraffin embedded (FFPE)
  • urine
  • faeces.

Post-extraction processing:

  • dilution and Normalisation
  • plating – 48, 96, 384 well formats
  • DNA fragmentation
  • PCR.

Sample QC:

  • Spectrophotomic (Nanodrop)
  • Fluorescence (Qubit, Picogreen)
  • Tapestation
  • Agarose Gel.

Our Team

Susan List-Armitage

Nadine Fitzpatrick

Jenny Quiatchon

Tabatha Goncalves


Equipment

Sample Processing

Revvity/Chemagic 360 : nucleic extraction automation (Volumes 200µL to 10mL

Biotek Synergy HT/H4: Spectrophotomic and fluorescence reading

Gallery